Journal: Oncology Letters

Article Title: Expression levels of the long noncoding RNA steroid receptor activator promote cell proliferation and invasion and predict patient prognosis in human cervical cancer

doi: 10.3892/ol.2018.9265

Figure Lengend Snippet: SRA promoted cell proliferation in SiHa cells. (A) Map of plasmid pLenti6/V5-D-TOPO and the sequence of the overexpression fusion gene SRA. (B) Overexpression of SRA in SiHa cells was analyzed using reverse transcription-quantitative polymerase chain reaction. (C) Cell proliferation was analyzed using Cell Counting kit-8 assays. Bars indicate the mean ± standard deviation of three independent experiments. *P<0.05 vs. SiHa, Vector cells. SD, standard deviation; SRA, steroid receptor activator.

Article Snippet: Cell lines and cell culture The human cervical squamous carcinoma SiHa cells was obtained from the Korean Cell Line Bank (Seoul, Korea) and provided by the Korea Gynecologic Cancer Bank through the Bio and Medical Technology Development Program of the Minister of Science, Information and Communication Technology and Future Planning, Korea.

Techniques: Plasmid Preparation, Sequencing, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Counting, Standard Deviation

Journal: Oncology Letters

Article Title: Expression levels of the long noncoding RNA steroid receptor activator promote cell proliferation and invasion and predict patient prognosis in human cervical cancer

doi: 10.3892/ol.2018.9265

Figure Lengend Snippet: SRA promoted cell migration and invasion. (A) Wound healing assay observed under the optical microscope were used to determine migration in SRA-overexpressing SiHa cells (×200). Cells after 24 and 48 h analyzed and determine for SiHa cells as a control. (B) Presents the wound healing assay percentages of each cell line. (C) Cell invasion observed under the optical microscope. Matrigel invasion assays were used to determine invasion after 48 h in SRA-overexpressing SiHa cells. (D) Presents the Matrigel invasion assay percentages of each cell line. Each assay was performed in triplicate. Data are means ± standard deviation. **P<0.01 vs. SiHa, Vector cells. ***P<0.0001 vs. SiHa, Vector cells. SRA, steroid receptor activator.

Article Snippet: Cell lines and cell culture The human cervical squamous carcinoma SiHa cells was obtained from the Korean Cell Line Bank (Seoul, Korea) and provided by the Korea Gynecologic Cancer Bank through the Bio and Medical Technology Development Program of the Minister of Science, Information and Communication Technology and Future Planning, Korea.

Techniques: Migration, Wound Healing Assay, Microscopy, Control, Invasion Assay, Standard Deviation, Plasmid Preparation

Journal: Oncology Letters

Article Title: Expression levels of the long noncoding RNA steroid receptor activator promote cell proliferation and invasion and predict patient prognosis in human cervical cancer

doi: 10.3892/ol.2018.9265

Figure Lengend Snippet: Effects of overexpression SRA on EMT-associated genes in SiHa cells. (A) Levels of E-cadherin, N-cadherin, Snail, β-catenin, Wnt5β, Vimentin and Twist were analyzed by reverse transcription-quantitative polymerase chain reaction in SRA overexpression SiHa cells. (B) Protein lysates were obtained from SRA-overexpressing SiHa cells. Levels of proteins in EMT-associated gene were analyzed using western blotting. (C) The band intensities were quantitated. The histogram revealed the average volume density corrected for the loading control (β-actin). *P<0.05 vs. SiHa cells. SRA, steroid receptor activator; EMT, epithelial-mesenchymal transition.

Article Snippet: Cell lines and cell culture The human cervical squamous carcinoma SiHa cells was obtained from the Korean Cell Line Bank (Seoul, Korea) and provided by the Korea Gynecologic Cancer Bank through the Bio and Medical Technology Development Program of the Minister of Science, Information and Communication Technology and Future Planning, Korea.

Techniques: Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: Gels

Article Title: Fluoride-Ion-Responsive Sol–Gel Transition in an L-Cysteine/AgNO 3 System: Self-Assembly Peculiarities and Anticancer Activity

doi: 10.3390/gels10050332

Figure Lengend Snippet: The cytotoxicity (MTT) of F − -based CSG against ( A ) SiHa and ( B ) Wi-38 cells at different anion concentrations (mM): 1 —3.1, 2 —3.5, 3 —3.9. The cytotoxicity (MTT) of SO 4 2− -based CSG ( 1 ), CSS ( 2 ) and NaF ( 3 ) against ( C ) SiHa and ( D ) Wi-38 cells. SiHa and Wi-38 cell incubation with systems took place for 48 h.

Article Snippet: CCL-75) and human squamous cervical carcinoma cells SiHa (ATCC, USA, Lot.

Techniques: Incubation

Journal: PLoS ONE

Article Title: Induction of apoptosis by pinostrobin in human cervical cancer cells: Possible mechanism of action

doi: 10.1371/journal.pone.0191523

Figure Lengend Snippet: Cytotoxicity % (CT) was assessed in cervical cell lines (A) HeLa, (B) Ca Ski, (C) SiHa cells on different concentrations of P N treatments as determined by MTT reduction assay at different incubation period. The bar graphs represent the percentage of cytotoxicity of P N in the cells. Cytotoxicity is shown as mean ± SD derived from at least three separate experiments in triplicate wells. Ordinary two-way ANOVA (multiple comparisons) was performed to calculate the statistical difference ( p≤0 . 05 ) among all treated groups as compared to vehicle treated group. * represents p ≤0.05, ∞ p ≤0.01, $ p ≤0.001 and # p ≤0.0001.

Article Snippet: HeLa (human adeno cervical carcinoma cells), Ca Ski (human epidermoid cervical carcinoma cells), SiHa (Grade II, squamous cervical carcinoma cell) and HEK293 (Human embryonic kidney cells) cell lines were procured from culture collection at National Centre for Cell Science (NCCS, Pune) India.

Techniques: MTT Reduction Assay, Incubation, Derivative Assay